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Fractions add up to the fresh monosomes, light, medium, and you may heavier polysomes had been pooled separately Powdered leftover ventricular center muscle (3 replicates for each congenic line) is lysed in polysome lysis buffer comprising 20 mM Hepes pH 7 5, 5 mM MgCl2, 300 mM KCl, 2 mM DTT, 100 ?g/mL cycloheximide, 0.2% […]

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